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通过酶联免疫吸附测定法检测兔血清中针对流感嗜血杆菌外膜囊泡的抗体

Antibody assay of rabbit sera for outer membrane vesicles of Haemophilus influenzae by enzyme-linked immunosorbent assay.

作者信息

Harada T, Sakakura Y, Yoshinaka Y

出版信息

Med Microbiol Immunol. 1985;174(4):197-203. doi: 10.1007/BF02123696.

DOI:10.1007/BF02123696
PMID:3877861
Abstract

Enzyme-linked immunosorbent assay (ELISA) was applied to the detection of IgG and IgM antibodies against outer membrane vesicles (OMV) antigen of Haemophilus influenzae type b. In this ELISA system, IgG antibody titers were about 40 fold higher than those in indirect hemagglutination assay (IHA). The IgG antibody titers by this ELISA of rabbit sera obtained after immunization were comparable with those by radioimmunoassay (RIA) of the same sera. A significant correlation was established between these two assays (r = 0.973, P less than 0.001).

摘要

采用酶联免疫吸附测定法（ELISA）检测抗b型流感嗜血杆菌外膜囊泡（OMV）抗原的IgG和IgM抗体。在该ELISA系统中，IgG抗体效价比间接血凝试验（IHA）中的效价高约40倍。免疫后获得的兔血清经此ELISA检测的IgG抗体效价与相同血清经放射免疫测定法（RIA）检测的效价相当。这两种检测方法之间建立了显著相关性（r = 0.973，P<0.001）。

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