Wetekam W, Groneberg J, Leineweber M, Wengenmayer F, Winnacker E L
Gene. 1982 Sep;19(2):179-83. doi: 10.1016/0378-1119(82)90004-x.
DNA complementary to preproinsulin messenger RNA from the primate Macaca fascicularis has been cloned into the PstI endonuclease site of the plasmid pBR322. One clone contains the entire preproinsulin coding region as well as 59 nucleotides of the 5'-untranslated region. The results predict an amino acid sequence for the Macaca fascicularis preproinsulin and establish for the first time that the primary structures of human and primate insulins are identical. The two amino acid exchanges between human and primate preproinsulins are restricted to the pre- and the C-peptide, respectively.
与灵长类猕猴前胰岛素原信使核糖核酸互补的DNA已被克隆到质粒pBR322的PstI核酸内切酶位点。一个克隆包含整个前胰岛素原编码区以及5'-非翻译区的59个核苷酸。这些结果预测了猕猴前胰岛素原的氨基酸序列，并首次确定人类和灵长类胰岛素的一级结构是相同的。人类和灵长类前胰岛素原之间的两个氨基酸交换分别局限于前肽和C肽。
