Hahn V, Winkler J, Rapoport T A, Liebscher D H, Coutelle C, Rosenthal S
Nucleic Acids Res. 1983 Jul 11;11(13):4541-52. doi: 10.1093/nar/11.13.4541.
The nucleic acid sequence of the preproinsulin cDNA of carp (Cyprinus carpio), cloned in the PstI-site of pBR322 (1), has been determined. The sequenced insert of 439 bp includes the complete coding information for carp preproinsulin (108 amino acids), 10 nucleotides of the 5'-and 105 nucleotides of the 3'-nontranslated regions. The nucleotide sequence confirms the previously established amino acid sequence of carp insulin (2) and determines those of the signal (21 aa 1) and C-peptide (35 aa 1). The observed shortness of the signal peptide of carp preproinsulin and the N-terminal addition of 2 amino acids to the carp insulin B-chain suggest that the cleavage site of the signal peptidase has moved. Calculations based on the comparison of known preproinsulin cDNA sequences showed that the evolutionary distance between fresh water and salt water teleostians is not smaller than between man and chicken.
已确定克隆于pBR322的PstI位点的鲤鱼(鲤属)前胰岛素原cDNA的核酸序列(1)。439bp的测序插入片段包含鲤鱼前胰岛素原(108个氨基酸)的完整编码信息、5'端的10个核苷酸和3'非翻译区的105个核苷酸。核苷酸序列证实了先前确定的鲤鱼胰岛素氨基酸序列(2),并确定了信号肽(21个氨基酸)和C肽(35个氨基酸)的序列。观察到鲤鱼前胰岛素原信号肽较短以及鲤鱼胰岛素B链N端添加了2个氨基酸,这表明信号肽酶的切割位点发生了移动。基于已知前胰岛素原cDNA序列比较的计算表明,淡水硬骨鱼和咸水硬骨鱼之间的进化距离不小于人和鸡之间的进化距离。