人胰岛素原前体互补DNA的核苷酸序列

Nucleotide sequence of human preproinsulin complementary DNA.

作者信息

Sures I, Goeddel D V, Gray A, Ullrich A

出版信息

Science. 1980 Apr 4;208(4439):57-9. doi: 10.1126/science.6927840.

Abstract

Recombinant bacterial plasmids that contain DNA complementary to human preproinsulin messenger RNA have been constructed. One clone contains the entire preproinsulin coding region, as well as the 3' untranslated region of the messenger RNA and eight nucleotides of the 5' untranslated region. Additional sequence information for the 5' untranslated region was obtained with the use of insulinoma messenger RNA in conjunction with specific primers from the cloned DNA for enzymatic chain termination sequence analysis. The results confirm the amino acid sequence of human proinsulin previously determined, and predict the amino acid sequence of the human preproinsulin signal peptide.

摘要

已构建出含有与人类胰岛素原信使核糖核酸互补的DNA的重组细菌质粒。一个克隆包含整个胰岛素原编码区，以及信使核糖核酸的3'非翻译区和5'非翻译区的八个核苷酸。通过使用胰岛素瘤信使核糖核酸结合来自克隆DNA的特异性引物进行酶促链终止序列分析，获得了5'非翻译区的额外序列信息。结果证实了先前确定的人类胰岛素原的氨基酸序列，并预测了人类胰岛素原信号肽的氨基酸序列。