Roelfzema H, van Erp P E
J Invest Dermatol. 1983 Jan;80(1):24-6. doi: 10.1111/1523-1747.ep12530949.
Quantification and characterization of [3H]-fucose-labeled cell surface glycoproteins are reported. Two approaches have been compared; first the analysis of glycoprotein shed spontaneously into the medium during incubation of keratinocytes in vitro, and second the study of material released by exposure of the cell to proteolytic enzymes. It is shown that psoriatic keratinocytes "shed" glycoprotein more rapidly than normal, although the material is of similar molecular weight (mainly "biantennary" transferrin type glycopeptides). By contrast, the percentage of glycoprotein released by proteolysis of psoriatic keratinocytes is normal, but the molecular weight distribution of the labeled glycopeptides is markedly altered. The abnormal turnover and composition of fucose-labeled glycoproteins from the cell surface may be related to the loss of growth control in psoriatic epidermis.
本文报道了对[3H] - 岩藻糖标记的细胞表面糖蛋白的定量和表征。比较了两种方法：一是分析角质形成细胞在体外培养期间自发释放到培养基中的糖蛋白，二是研究细胞暴露于蛋白水解酶后释放的物质。结果表明，银屑病角质形成细胞“释放”糖蛋白的速度比正常细胞快，尽管释放物质的分子量相似（主要是“双天线”转铁蛋白型糖肽）。相比之下，银屑病角质形成细胞经蛋白水解后释放的糖蛋白百分比正常，但标记糖肽的分子量分布明显改变。细胞表面岩藻糖标记糖蛋白的异常周转和组成可能与银屑病表皮生长控制的丧失有关。
