Harzmann R, Löwer J, Löwer R, Bichler K H, Kurth R
J Urol. 1982 Nov;128(5):1055-9. doi: 10.1016/s0022-5347(17)53343-1.
Two lines of evidence led to the investigation of human teratocarcinoma cells in vitro for oncogenic retroviruses: the observation by electron microscopy of retrovirus-like particles budding from the syncytial trophoblasts of human placentas, and the demonstration that teratocarcinoma patients before treatment show a high serum antibody reactivity against envelope proteins of mammalian retroviruses. In all 5 teratocarcinoma cell lines studied so far, retrovirus-like particles have been detected by electron microscopy. The production of these human teratocarcinoma-derived (HTD)-particles is enhanced by induction procedures known to be effective in animal virus model systems. In parallel, virus induction also increases the level of chorionic gonadotropin in the culture supernatant, demonstrating syncytial trophoblast-like cells in the heterogeneous cell population of teratocarcinomas which could be responsible for HTD-particle production. The origin of these virus-like particles--endogenous or exogenous--as well as their role in the pathogenesis of teratocarcinomas is not known so far. The potential value of serum antibody reactivity in teratocarcinoma patients as a diagnostic marker is discussed.
通过电子显微镜观察到从人胎盘的合体滋养层芽生出的逆转录病毒样颗粒，以及证明未经治疗的畸胎瘤患者血清对哺乳动物逆转录病毒包膜蛋白具有高抗体反应性。在迄今为止研究的所有5种畸胎瘤细胞系中，通过电子显微镜均检测到了逆转录病毒样颗粒。已知在动物病毒模型系统中有效的诱导程序可增强这些源自人畸胎瘤（HTD）颗粒的产生。同时，病毒诱导也会增加培养上清液中绒毛膜促性腺激素的水平，这表明畸胎瘤异质细胞群中存在合体滋养层样细胞，它们可能是HTD颗粒产生的原因。这些病毒样颗粒的起源——内源性还是外源性——以及它们在畸胎瘤发病机制中的作用目前尚不清楚。本文还讨论了畸胎瘤患者血清抗体反应性作为诊断标志物的潜在价值。
