Sorg C
Mol Immunol. 1982 Oct;19(10):1275-8. doi: 10.1016/0161-5890(82)90293-0.
Macrophages which are intimately involved in acute and chronic inflammatory reactions are functionally heterogeneous not only with regard to the expression of constitutive functions but also in their response to lymphokine signals. The biological basis of this heterogeneity is poorly understood. Whether we are dealing with true subpopulations or with intermediately stable phenotypes has not been resolved. To study these questions we adopted a bone marrow liquid culture system in which bone marrow cells--in the presence of a colony-stimulating factor--proliferate and differentiate into macrophages. This culture system was taken here as a model to study the expression of various functions by macrophages in the course of maturation. Several tests were performed daily and in parallel from the same batch of cells. It was found that certain functions were expressed early and were also characteristic for mature macrophages such as Fc receptors, phagocytosis of latex beads and unspecific esterase. Other functions appeared and disappeared in an ordered sequence, such as the response to macrophage migration inhibitory factor and chemotactic factor as well as the production of interferon and plasminogen activator. The time course of functional expression was strongly dependent on proliferation of precursor cells as well as on proliferation of differentiated macrophages. It is concluded that the transient phenotypic expression of functions during differentiation is the basis for the functional heterogeneity of macrophages.
巨噬细胞密切参与急性和慢性炎症反应，其功能不仅在组成性功能的表达方面存在异质性，而且在对淋巴因子信号的反应方面也存在异质性。这种异质性的生物学基础目前还知之甚少。我们面对的究竟是真正的亚群还是处于中间稳定状态的表型，这一问题尚未得到解决。为了研究这些问题，我们采用了一种骨髓液体培养系统，在该系统中，骨髓细胞在集落刺激因子的存在下增殖并分化为巨噬细胞。在此，这个培养系统被用作研究巨噬细胞在成熟过程中各种功能表达的模型。每天从同一批细胞中并行进行多项测试。结果发现，某些功能在早期就已表达，并且也是成熟巨噬细胞的特征，如Fc受体、乳胶珠吞噬作用和非特异性酯酶。其他功能则按顺序出现和消失，如对巨噬细胞移动抑制因子和趋化因子的反应以及干扰素和纤溶酶原激活物的产生。功能表达的时间进程强烈依赖于前体细胞的增殖以及分化巨噬细胞的增殖。得出的结论是，分化过程中功能的短暂表型表达是巨噬细胞功能异质性的基础。
