Newhall W J, Batteiger B, Jones R B
Infect Immun. 1982 Dec;38(3):1181-9. doi: 10.1128/iai.38.3.1181-1189.1982.
Sera from individuals with culture-proven genital infection with Chlamydia trachomatis were analyzed for the presence of antibodies to chlamydial proteins by an immunoelectrophoretic transfer method. Protein antigens from representative strains of the 15 known serotypes were resolved by gel electrophoresis and transferred to a nitrocellulose solid support before being probed with serum. Sera from infected patients reacted with many different proteins. Most of these sera reacted with a 60,000- and a 62,000-molecular-weight protein which were present in each of the C. trachomatis serotypes and clinical isolates analyzed. In contrast, reactions with the major outer membrane protein were frequently observed but were usually weak. Sera from control groups of children, cloistered nuns, and college women, who were presumed not to have had prior chlamydial infections, did not usually have antibodies against the 60,000- or 62,000-molecular-weight protein, but did react with the major outer membrane protein and a 29,000-molecular-weight protein. These observations may have implications for the development of serodiagnostic tests as well as the identification of candidate antigens for vaccine development.
采用免疫电泳转移法，对经培养证实感染沙眼衣原体的个体血清进行分析，以检测抗衣原体蛋白抗体的存在。将15种已知血清型代表性菌株的蛋白质抗原进行凝胶电泳分离，然后转移至硝酸纤维素固相支持物上，再用血清进行检测。感染患者的血清与许多不同蛋白质发生反应。这些血清大多与分子量为60,000和62,000的蛋白质发生反应，这两种蛋白质存在于所分析的每种沙眼衣原体血清型和临床分离株中。相比之下，虽经常观察到与主要外膜蛋白的反应，但通常较弱。来自儿童、隐居修女和大学女生对照组的血清，推测这些人之前未感染过衣原体，通常没有针对分子量为60,000或62,000蛋白质的抗体，但确实与主要外膜蛋白和一种分子量为29,000的蛋白质发生反应。这些观察结果可能对血清诊断试验的开发以及疫苗开发候选抗原的鉴定具有重要意义。
