Department of Pathobiology, School of Public Health and Community Medicine, University of Washington, Seattle, Washington 98195.
Infect Immun. 1973 Mar;7(3):356-60. doi: 10.1128/iai.7.3.356-360.1973.
Responding to the pressure of large numbers of trachoma-inclusion conjunctivitis (TRIC)-lymphogranuloma venereum (LGV) isolates from field studies requiring serotyping, we have developed a simplified, less-precise method that utilizes cell culture-grown organisms to produce mouse antisera which is tested agaist prototype TRIC-LGV antigens in the micro-immunofluorescence test. Cell cultures with as few as 5 to 15% of cells showing inclusions produced adequate antibody in mice 4 days after single injection. Knowledge of the reaction of prototype antisera with the antigens has allowed typing of most isolates tested from the pattern of cross-reaction of their antiserum.
为应对大量沙眼包涵体结膜炎（TRIC）-性病淋巴肉芽肿（LGV）分离株的压力，这些分离株来自需要血清分型的现场研究，我们开发了一种简化、不那么精确的方法，该方法利用细胞培养物中生长的生物体来产生小鼠抗血清，然后在微量免疫荧光试验中检测其与原型 TRIC-LGV 抗原的反应。在单次注射后 4 天，细胞培养物中只要有 5%至 15%的细胞出现包涵体，就可以在小鼠中产生足够的抗体。对原型抗血清与抗原的反应的了解允许对从血清交叉反应模式测试的大多数分离株进行分型。
