Crerar M M, Swain W F, Pictet R L, Nikovits W, Rutter W J
J Biol Chem. 1983 Jan 25;258(2):1311-7.
Portions of at least nine distinct rat amylase genes or pseudogenes have been isolated. Cloned rat genomic DNA fragments containing complete or major portions of seven of these have been examined by heteroduplex analysis and fall within two separate groups based on their degree of homology. Four gene sequences comprising one of these groups are closely related to pancreatic amylase mRNA. The other group shows significant nonhomology to both pancreatic and parotid amylase cDNAs and may represent an additional gene type(s). All of the cloned amylase gene sequences are found in rat genomic DNA. Additional amylase sequences which have not yet been cloned are also detected. Comparison of DNA from individual Sprague-Dawley rats by Southern blotting techniques indicates allelic variation at multiple amylase loci.
至少九个不同的大鼠淀粉酶基因或假基因的部分已被分离出来。含有其中七个完整或主要部分的克隆大鼠基因组DNA片段已通过异源双链分析进行检测，并根据它们的同源程度分为两个不同的组。构成其中一组的四个基因序列与胰腺淀粉酶mRNA密切相关。另一组与胰腺和腮腺淀粉酶cDNA均无明显同源性，可能代表一种额外的基因类型。所有克隆的淀粉酶基因序列都存在于大鼠基因组DNA中。尚未克隆的其他淀粉酶序列也被检测到。通过Southern印迹技术对个体斯普拉-道莱大鼠的DNA进行比较，表明多个淀粉酶基因座存在等位基因变异。
