Strum J M, Hall-Craggs E C
J Neurosci Methods. 1982 Nov;6(4):305-9. doi: 10.1016/0165-0270(82)90031-0.
The cytochemical localization of acetylcholinesterase activity at motor endplates is commonly employed prior to electron microscopic examination. Methods which involve the use of thiocholine esters often lead to a loss of resolution due to the spread to surrounding tissues of an extremely dense reaction product. A method is presented in which esterase positive sites are revealed by incubation with hexazotized pararosaniline and indoxyl acetate. The osmiophilic property of the reaction product which remains localized to the synaptic cleft allows speedy identification and detailed examination of the endplate at the ultrastructural level. An application of the procedure is also described.
在进行电子显微镜检查之前，通常会对运动终板处的乙酰胆碱酯酶活性进行细胞化学定位。由于硫代胆碱酯类物质会导致极密集的反应产物扩散到周围组织，涉及使用硫代胆碱酯类的方法常常会导致分辨率降低。本文介绍了一种方法，通过与重氮显色副蔷薇苯胺和乙酸吲哚酚孵育来显示酯酶阳性位点。反应产物的嗜锇特性使其保留在突触间隙，这有助于在超微结构水平上快速识别和详细检查终板。本文还描述了该方法的应用。
