Weyer A, Dandeu J P, Marchand F, David B
Ann Immunol (Paris). 1982 Jul-Aug;133D(1):87-94.
Histamine release experiments were performed over a 10(6)-fold range of allergen concentrations on basophils of patients sensitive to Dermatophagoides farinae. Two extracts of D. farinae were used, a partially purified one (Df 80d) containing several allergens and a highly purified component (Ag11). This histamine release patterns obtained with the complex allergen mixture Df 80d gave broad histamine release curves presenting an initial increase, followed by a decrease or a plateau, with occasionally a small dip. The Ag11-induced histamine release curves presented two peaks (bimodal pattern), separated by a more or less pronounced inhibition dip. No non-specific histamine releasing activity was observed with the basophils of two controls. The possible theoretical models for the bimodal histamine release patterns are discussed.
在对粉尘螨敏感患者的嗜碱性粒细胞上，针对浓度范围达10⁶倍的变应原进行了组胺释放实验。使用了两种粉尘螨提取物，一种是含有多种变应原的部分纯化提取物（Df 80d），另一种是高度纯化的成分（Ag11）。用复合变应原混合物Df 80d获得的组胺释放模式呈现出宽泛的组胺释放曲线，最初上升，随后下降或趋于平稳，偶尔有小幅度下降。Ag11诱导的组胺释放曲线呈现出两个峰值（双峰模式），中间被或多或少明显的抑制性下降隔开。两名对照者的嗜碱性粒细胞未观察到非特异性组胺释放活性。讨论了双峰组胺释放模式可能的理论模型。
