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多线染色体中染色体转录本的原位免疫荧光可视化。

In situ immunofluorescent visualization of chromosomal transcripts in polytene chromosomes.

作者信息

Alcover A, Izquierdo M, Stollar D, Kitagawa Y, Miranda M, Alonso C

出版信息

Chromosoma. 1982;87(3):263-77. doi: 10.1007/BF00327629.

DOI:10.1007/BF00327629
PMID:6186443
Abstract

The induction and distribution of chromosomal transcripts in the polytene chromosomes of D. melanogaster and D. hydei has been investigated by indirect immunofluorescence using an antiserum directed against DNA/RNA hybrids. The fluorescence was intense and occurred in most of the chromosomal subdivisions when the chromosomes were exposed to denaturing conditions and then allowed to reanneal. The extent of hybrid formation depended both on the extent of DNA denaturation and on the maintenance of RNA integrity. Fluorescence was absent from chromosomes treated with pancreatic RNase before denaturation. The velocity of the chromosomal DNA/RNA hybridization reaction and the effects of the initiation inhibitor of RNA synthesis, DRB, suggest that in order to hybridize the RNA has to be located in its transcriptional compartment. Even though overall patterns of fluorescence seem to be similar during a developmental stage, variations were observed, particularly some correlated with puff induction after ecdysone stimulation.

摘要

利用针对DNA/RNA杂交体的抗血清，通过间接免疫荧光法研究了黑腹果蝇和海德氏果蝇多线染色体中染色体转录本的诱导和分布。当染色体暴露于变性条件下然后复性时，荧光强烈且出现在大多数染色体亚区。杂交形成的程度既取决于DNA变性的程度，也取决于RNA完整性的维持。变性前用胰核糖核酸酶处理的染色体没有荧光。染色体DNA/RNA杂交反应的速度以及RNA合成起始抑制剂DRB的作用表明，为了进行杂交，RNA必须位于其转录区域。尽管在发育阶段荧光的总体模式似乎相似，但仍观察到了变化，特别是一些与蜕皮激素刺激后胀泡诱导相关的变化。

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In situ immunofluorescent visualization of chromosomal transcripts in polytene chromosomes.多线染色体中染色体转录本的原位免疫荧光可视化。
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Immunofluorescent characterization of DNA . RNA hybrids on polytene chromosomes of Trichosia pubescens (Diptera, sciaridae).毛眼蕈蚊（双翅目，眼蕈蚊科）多线染色体上DNA.RNA杂交体的免疫荧光特征分析
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Polytene chromosome puffing and in situ hybridization measure different aspects of RNA metabolism.多线染色体胀泡和原位杂交检测RNA代谢的不同方面。
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Immunofluorescence localization of DNA:RNA hybrids in Drosophila melanogaster polytene chromosomes.黑腹果蝇多线染色体中DNA:RNA杂交体的免疫荧光定位
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The ultrastructural morphology of native salivary gland chromosomes of Drosophila melanogaster: the band-interband question.黑腹果蝇天然唾液腺染色体的超微结构形态：带间带问题
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Analytical studies of chromosomal transcription detected by an endogenous hybridization technique (EHT) using autoradiography and indirect immunofluorescence in Drosophila hydei.利用放射自显影和间接免疫荧光技术，通过内源性杂交技术（EHT）对海德氏果蝇染色体转录进行的分析研究。
Acta Embryol Morphol Exp (Halocynthia Assoc). 1981 Dec;2(2):131-40.
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RNA polymerase B in polytene chromosomes: immunofluorescent and autoradiographic analysis during stimulated and repressed RNA synthesis.多线染色体中的RNA聚合酶B：刺激和抑制RNA合成过程中的免疫荧光和放射自显影分析。
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Salivary gland function and chromosomal puffing patterns in Drosophila hydei.海德果蝇的唾液腺功能与染色体疏松模式
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Molecular hybridization of radioactive DNA to the DNA of cytological preparations.放射性DNA与细胞学制剂的DNA进行分子杂交。
Proc Natl Acad Sci U S A. 1969 Oct;64(2):600-4. doi: 10.1073/pnas.64.2.600.
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