Vlassova I E, Umbetova G H, Zimmermann V H, Alonso C, Belyaeva E S, Zhimulev I F
Chromosoma. 1985;91(3-4):251-8. doi: 10.1007/BF00328220.
Sites of transcriptional activity in the whole set of Drosophila melanogaster polytene chromosomes have been localized by means of fluorescent antibodies against DNA:RNA hybrid molecules and compared with results on 3H-uridine incorporation obtained earlier. The majority of large and small puffs with intensive 3H-uridine incorporation demonstrate bright fluorescence. Moreover, bright fluorescence is also observed for a large number of small puffs though the intensity of 3H-uridine incorporation is low. Some prominent puffs with high levels of 3H-uridine incorporation show weak fluorescence. Condensed bands, as a rule, do not show fluorescence. The regions that look like interbands under the light microscope are not real interbands, but consist of minibands visible only in the electron microscope (EM). However, a region that has been previously studied by EM and proven to be a real interband between two thick dark bands (100B3-100B4-5) showed fluorescence. These data support previous suggestions indicating a substantial contribution of transcriptional products from small puffs and interbands to the whole transcriptional system of polytene chromosomes.
利用针对DNA:RNA杂交分子的荧光抗体，已确定了黑腹果蝇整套多线染色体中转录活性的位点，并将其与早期获得的3H-尿苷掺入结果进行了比较。大多数大量掺入3H-尿苷的大、小胀泡显示出明亮的荧光。此外，尽管3H-尿苷掺入强度较低，但仍有大量小胀泡观察到明亮的荧光。一些大量掺入3H-尿苷的显著胀泡显示出较弱的荧光。通常，浓缩带不显示荧光。在光学显微镜下看起来像间带的区域并非真正的间带，而是由仅在电子显微镜（EM）下可见的微带组成。然而，一个先前经EM研究并被证明是两条粗暗带（100B3 - 100B4 - 5）之间真正间带的区域显示出荧光。这些数据支持了先前的推测，即小胀泡和间带的转录产物对多线染色体的整个转录系统有重大贡献。
