Poulsen H S
Eur J Cancer Clin Oncol. 1982 Nov;18(11):1075-9. doi: 10.1016/0277-5379(82)90086-4.
Samples of human breast cancer tissue were analyzed for estrogen receptor content (ER) with a hydroxylapatite method (HAP) as well as a dextran-coated charcoal method (DCC). It was found that both methods revealed artificially low ER concentrations in cytosols with low protein concentration. It was also found that the DCC assay underestimated the ER activity in the tumors compared to the HAP assay. No differences in terms of low affinity binding nor Kd-values of high affinity estrogen binding were observed. A significant higher background radioactivity was obtained in the HAP assay, and in some tumors one assay would classify them as ER-negative and another as ER-positive.
采用羟基磷灰石法（HAP）和葡聚糖包被活性炭法（DCC）对人乳腺癌组织样本的雌激素受体含量（ER）进行分析。结果发现，两种方法均显示，在蛋白质浓度较低的胞质溶胶中，ER浓度人为降低。还发现，与HAP法相比，DCC检测低估了肿瘤中的ER活性。在低亲和力结合以及高亲和力雌激素结合的解离常数（Kd值）方面未观察到差异。HAP检测获得的背景放射性显著更高，在某些肿瘤中，一种检测方法将其归类为ER阴性，而另一种检测方法则将其归类为ER阳性。
