Goldman J E
J Neuropathol Exp Neurol. 1983 Mar;42(2):146-52. doi: 10.1097/00005072-198303000-00004.
Ultrastructural studies of the rod-shaped eosinophilic inclusions of the hippocampus (Hirano bodies) have demonstrated thin, filamentous components. The size of the filaments suggests that actin polymers (microfilaments) might form part of the Hirano body. To investigate this possibility, sections of human hippocampus and neocortex were stained, using a peroxidase-antiperoxidase (PAP) technique, with an antiserum against actin. Dense reaction product was seen over rod-shaped bodies, the location and morphology of which were typical of Hirano bodies. Immunocytochemical reactions on tissue previously stained with hematoxylin and eosin allowed a direct comparison between Hirano bodies, identified by their shape and eosinophilia, and the PAP reaction product. These results suggest an abnormal organization of a major cytoskeletal protein in hippocampal neurons, especially those of aged brains.
对海马体中杆状嗜酸性包涵体（ Hirano小体）的超微结构研究已证实其存在细的丝状成分。这些细丝的大小表明肌动蛋白聚合物（微丝）可能是Hirano小体的一部分。为了研究这种可能性，采用过氧化物酶 - 抗过氧化物酶（PAP）技术，用人抗肌动蛋白抗血清对人海马体和新皮质切片进行染色。在杆状体上可见密集的反应产物，其位置和形态是Hirano小体的典型特征。对先前用苏木精和伊红染色的组织进行免疫细胞化学反应，可以直接比较通过形状和嗜酸性确定的Hirano小体与PAP反应产物。这些结果表明海马神经元中主要细胞骨架蛋白存在异常组织，尤其是老年大脑中的神经元。
