Mitake S, Katada E, Otsuka Y, Matsukawa N, Iwase T, Tsugu T, Fujimori O, Ojika K
Second Department of Internal Medicine, Medical School, Nagoya City University, Japan.
Neuropathol Appl Neurobiol. 1996 Oct;22(5):440-5. doi: 10.1111/j.1365-2990.1996.tb00918.x.
We have previously demonstrated that hippocampal cholinergic neurostimulating peptide (HCNP)-related components accumulate in almost all Hirano bodies in Sommer's sector of the hippocampus of elderly individuals, and that the number of HCNP-positive Hirano bodies is greater in patients with Alzheimer's disease. Although Hirano bodies occur preferentially in the neuronal processes of the stratum pyramidale of the hippocampus, they can be seen occasionally as small inclusions, intermingled with neurofibrillary tangles and in association with senile plaques. Here we show that the small inclusions are also recognized by an anti-HCNP antibody, and by using immunoelectron microscopy demonstrate that these HCNP-positive inclusions, intermingled with tau protein-positive neurofibrillary tangles and beta-amyloid-positive senile plaques are indeed Hirano bodies. These findings strongly suggest that HCNP-related components may be involved in Hirano body formation.
我们之前已经证明，海马胆碱能神经刺激肽（HCNP）相关成分几乎在所有老年人海马Sommer区的 Hirano 小体中积累，并且在阿尔茨海默病患者中，HCNP 阳性的 Hirano 小体数量更多。尽管 Hirano 小体优先出现在海马锥体层的神经元突起中，但它们偶尔也可见为小的包涵体，与神经原纤维缠结混合并与老年斑相关。在这里，我们表明这些小包涵体也能被抗 HCNP 抗体识别，并且通过免疫电子显微镜证明，这些与 tau 蛋白阳性的神经原纤维缠结和β-淀粉样蛋白阳性的老年斑混合的 HCNP 阳性包涵体确实是 Hirano 小体。这些发现强烈表明，HCNP 相关成分可能参与了 Hirano 小体的形成。
