Jordan-Sciutto K, Dragich J, Walcott D, Bowser R
Department of Pathology, University of Pittsburgh School of Medicine, PA 15261, USA.
Neuropathol Appl Neurobiol. 1998 Oct;24(5):359-66. doi: 10.1046/j.1365-2990.1998.00140.x.
We have previously reported that the FAC1 protein is contained in hippocampal structures that resemble Hirano bodies. Hirano bodies are cytoplasmic inclusions containing actin filaments that are numerous in the hippocampus of many Alzheimer's disease patients. FAC1 is a developmentally regulated protein that is localized to the cytoplasm of neurons during development and is predominately a nuclear protein in adult brain. In hippocampal sections from non-demented adults. Alzheimer's disease, and dementia with Lewy bodies patients. Hirano bodies were immunolabelled with antibodies to the FAC1 protein. Confocal laser microscopy demonstrated the presence of actin in FAC1 labelled Hirano bodies, and ultrastructural analysis confirmed the presence of a lattice structure within FAC1 labelled Hirano bodies. Numerous FAC1 immunoreactive swollen dendrites were also present in the hippocampus of Alzheimer's disease and dementia with Lewy bodies patients. Within any one case the total number of FAC1 positive swollen dendrites correlated with the total number of Hirano bodies, suggesting an association between the two structures. Thus, FAC1 protein is contained in Hirano bodies and swollen dendrites in the hippocampus of patients with Alzheimer's disease and dementia with Lewy bodies.
我们之前曾报道，FAC1蛋白存在于类似于 Hirano 小体的海马结构中。Hirano 小体是含有肌动蛋白丝的细胞质内含物，在许多阿尔茨海默病患者的海马中大量存在。FAC1 是一种受发育调控的蛋白，在发育过程中定位于神经元的细胞质，在成体大脑中主要是一种核蛋白。在非痴呆成年人、阿尔茨海默病患者以及路易体痴呆患者的海马切片中，Hirano 小体用抗 FAC1 蛋白的抗体进行免疫标记。共聚焦激光显微镜显示在 FAC1 标记的 Hirano 小体中有肌动蛋白存在，超微结构分析证实了在 FAC1 标记的 Hirano 小体中有晶格结构。在阿尔茨海默病患者和路易体痴呆患者的海马中也存在大量 FAC1 免疫反应性肿胀树突。在任何一个病例中，FAC1 阳性肿胀树突的总数与 Hirano 小体总数相关，表明这两种结构之间存在关联。因此，FAC1 蛋白存在于阿尔茨海默病患者和路易体痴呆患者海马中的 Hirano 小体和肿胀树突中。
