Sauer B
J Microsc. 1983 Jan;129(Pt 1):75-87. doi: 10.1111/j.1365-2818.1983.tb04162.x.
A new semi-automatic method is introduced which makes possible the quantitative examination of cresyl-fast-violet stained sections of the cerebral cortex. These sections are divided into measuring fields of 20 X 20 microns in a well-defined area. A scanning procedure then automatically determines the grey level index of every single field. The image of the histological section can be compared with the grey level index data matrix by means of a plotted image, the density of the plotted points of which is proportional to the measured grey level index. By overlaying this plotted image with the original section, the boundaries of the laminae can be fixed in the data matrix. The grey level index profiles--the grey level data plot as a function of the cortical depth--after smoothing and standardization are typical of special cortical areas. The mean thickness and the mean portion of the grey level index profile of the different laminae of the striate area are compared with data from other publications.
本文介绍了一种新的半自动方法，该方法能够对经甲酚紫染色的大脑皮质切片进行定量检测。这些切片在一个定义明确的区域内被划分为20×20微米的测量区域。然后，通过扫描程序自动确定每个测量区域的灰度指数。组织学切片的图像可以通过绘制图像与灰度指数数据矩阵进行比较，绘制图像上的点的密度与测量的灰度指数成正比。通过将此绘制图像与原始切片叠加，可以在数据矩阵中确定各层的边界。经过平滑和标准化处理后的灰度指数剖面图（灰度数据随皮质深度的变化曲线）是特定皮质区域的典型特征。将纹状区不同层的平均厚度和灰度指数剖面图的平均部分与其他出版物的数据进行了比较。
