一种用气相色谱法测定血清中草酸盐的新方法。

A new method for determination of oxalate in blood serum by gas chromatography.

作者信息

Nuret P, Offner M

出版信息

Clin Chim Acta. 1978 Jan 2;82(1-2):9-12. doi: 10.1016/0009-8981(78)90020-7.

Abstract

A method for the determination of blood oxalate by gas chromatography is described. After clarification of the serum with acetone, the oxalate was extracted, ethylated and analysed on a gas chromatograph. The concentration of oxalate in the sample was determined by comparison with the elution pattern of diethylmalonate used as an internal standard. Using this technique, the recovery of added oxalate was more than 80%. Normal subjects were found to have blood oxalate levels of 27 mumol/l or less.

摘要

描述了一种通过气相色谱法测定血草酸的方法。用丙酮使血清澄清后，提取草酸，进行乙基化并在气相色谱仪上分析。通过与用作内标的丙二酸二乙酯的洗脱模式比较来测定样品中草酸的浓度。使用该技术，添加草酸的回收率超过80%。发现正常受试者的血草酸水平为27微摩尔/升或更低。