Wieringa T, de Bruin G, van Meerwijk W P, Krans H M
Cell Biophys. 1982 Dec;4(4):261-71. doi: 10.1007/BF02788313.
The influence of alterations in phospholipid structure by phospholipase treatment on insulin action and glucose transport in rat adipocytes was studied. It appeared that phospholipase A2 from bee venom caused a breakdown of approximately 50% of phosphotidylcholine without lysis of the cells. Because of this treatment, insulin binding was increased, resulting in an increased sensitivity of glucose transport towards lower insulin concentrations. Moreover, an increased affinity of the transport system for 2-deoxyglucose was observed. Phospholipase C from Clostridium welchii caused complete lysis of adipocytes. Phospholipase A2 from Crotalus adamenteus was without effect.
研究了用磷脂酶处理改变磷脂结构对大鼠脂肪细胞胰岛素作用和葡萄糖转运的影响。结果显示，蜂毒中的磷脂酶A2可使约50%的磷脂酰胆碱分解，而细胞不发生裂解。经此处理后，胰岛素结合增加，导致葡萄糖转运对较低胰岛素浓度的敏感性增加。此外，还观察到转运系统对2-脱氧葡萄糖的亲和力增加。产气荚膜梭菌的磷脂酶C可使脂肪细胞完全裂解。矛头蝮蛇的磷脂酶A2则无作用。
