Qian Z L, Deelder A M
Exp Parasitol. 1983 Apr;55(2):168-78. doi: 10.1016/0014-4894(83)90011-5.
The antigenic constituents of a trichloroacetic acid (TCA)-soluble fraction of adult Schistosoma japonicum were studied with immunoelectrophoresis, and compared with those of Schistosoma mansoni. Eight TCA-soluble antigens of S. japonicum were demonstrated, five of which showed immunological identity with S. mansoni antigens. Of the eight antigens, five antigens with anodic motility were found as circulating antigens in S. japonicum-infected hamster and rabbit sera; the major circulating antigen was the circulating anodic antigen (CAA). Two other antigens, with cathodic motility, including the circulating cathodic antigen (CCA), were demonstrable as circulating antigens in S. mansoni infections, but not in S. japonicum infections. Most of the circulating antigens were shown to be gut-associated. Only one antigen, line 2, which was not demonstrable as circulating antigen and which was present in the parenchyma of the worms, was found to be specific for S. japonicum. Using an ELISA for the detection of CAA in the sera of S. japonicum-infected rabbits, a lower detection level of 100 ng CAA/ml serum was achieved. Moreover, at 7-8 weeks after infection, a direct relationship between worm burden and CAA level was demonstrated.
用免疫电泳法研究了日本血吸虫成虫三氯乙酸（TCA）可溶性组分的抗原成分，并与曼氏血吸虫的抗原成分进行了比较。结果显示日本血吸虫有8种TCA可溶性抗原，其中5种与曼氏血吸虫抗原具有免疫同一性。在这8种抗原中，发现有5种具有阳极泳动性的抗原作为循环抗原存在于感染日本血吸虫的仓鼠和兔血清中；主要的循环抗原是循环阳极抗原（CAA）。另外两种具有阴极泳动性的抗原，包括循环阴极抗原（CCA），在曼氏血吸虫感染中可作为循环抗原检测到，但在日本血吸虫感染中未检测到。大多数循环抗原显示与肠道相关。只有一种抗原（第2条带），在感染兔血清中未作为循环抗原检测到，且存在于虫体实质中，被发现是日本血吸虫特有的。使用酶联免疫吸附测定法（ELISA）检测日本血吸虫感染兔血清中的CAA，检测下限达到100 ng CAA/ml血清。此外，在感染后7 - 8周，证明虫负荷与CAA水平之间存在直接关系。
