Nogueira-Queiroz J A, Lutsch C, Capron M, Dessaint J P, Capron A
Clin Exp Immunol. 1986 Aug;65(2):223-31.
Monoclonal antibodies were obtained after immunization of mice with Schistosoma mansoni excretory/secretory antigen, previously shown to contain the circulating cathodic (M) antigen. Among these, the 40:B1 monoclonal antibody proved to be specific for the schistosome genus and to detect only adult worm-derived antigens as shown both by immunoprecipitation and with a two-site immunoradiometric assay using the monoclonal as both the solid-phase and the labelled antibody. The two-site immunoradiometric assay allows a sensitive measurement (detection limit: 5 ng) of circulating schistosome antigen in blood and in urine from patients with schistosomiasis. The amount of circulating schistosome M antigen is correlated with schistosome egg excretion in stool.
用曼氏血吸虫排泄/分泌抗原免疫小鼠后获得了单克隆抗体，先前已证明该抗原含有循环阴极（M）抗原。其中，40:B1单克隆抗体被证明对血吸虫属具有特异性，并且如免疫沉淀以及使用该单克隆抗体作为固相和标记抗体的双位点免疫放射分析所示，仅检测成虫来源的抗原。双位点免疫放射分析能够灵敏地检测血吸虫病患者血液和尿液中的循环血吸虫抗原（检测限：5 ng）。循环血吸虫M抗原的量与粪便中血吸虫卵的排泄量相关。
