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人胎盘妊娠特异性β1-糖蛋白(SP1)的体外翻译

In vitro translation of human placental pregnancy-specific beta 1-glycoprotein (SP1).

作者信息

Hirunyavasit W, Kusamran T

出版信息

Experientia. 1983 Feb 15;39(2):206-7. doi: 10.1007/BF01958903.

DOI:10.1007/BF01958903
PMID:6187598
Abstract

Synthesis of SP1-glycoprotein by the human placenta was directly demonstrated, by in vitro translation of RNA extracted from full term and from early placentas in a cell-free wheat germ system followed by specific immunoprecipitation of the radioactively labeled nascent peptides. De novo synthesized SP1-glycoprotein in both RNA preparations accounted for 1-1.3% of total protein synthesis.

摘要

通过在无细胞小麦胚芽系统中对从足月胎盘和早期胎盘提取的RNA进行体外翻译,随后对放射性标记的新生肽进行特异性免疫沉淀,直接证明了人胎盘合成SP1糖蛋白。两种RNA制剂中从头合成的SP1糖蛋白占总蛋白合成的1-1.3%。

相似文献

1
In vitro translation of human placental pregnancy-specific beta 1-glycoprotein (SP1).人胎盘妊娠特异性β1-糖蛋白(SP1)的体外翻译
Experientia. 1983 Feb 15;39(2):206-7. doi: 10.1007/BF01958903.
2
Identification and molecular weight of SP1 synthesized from mRNA of human placenta in a wheat germ cell-free system.在麦胚无细胞系统中从人胎盘mRNA合成的SP1的鉴定及分子量测定
Mol Biol Rep. 1987;12(1):55-9. doi: 10.1007/BF00580651.
3
Cultured human syncytiotrophoblasts synthesize pregnancy-specific beta-1-glycoprotein (SP1).培养的人合体滋养层细胞合成妊娠特异性β-1-糖蛋白(SP1)。
Acta Pathol Microbiol Scand C. 1981 Apr;89(2):139-44. doi: 10.1111/j.1699-0463.1981.tb02677.x.
4
Characterization of pregnancy-specific beta 1-glycoprotein synthesized by human placental fibroblasts.人胎盘成纤维细胞合成的妊娠特异性β1-糖蛋白的特性分析。
Mol Endocrinol. 1989 Jan;3(1):89-96. doi: 10.1210/mend-3-1-89.
5
[Placental proteins and protein hormones in high risk pregnancies].
Glas Srp Akad Nauka Med. 2002(47):1-19.
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The Production of beta 1-specific pregnancy glycoprotein (SP1) by the amnion.羊膜产生β1特异性妊娠糖蛋白(SP1)。
Eur J Obstet Gynecol Reprod Biol. 1981 Feb;11(4):215-9. doi: 10.1016/0028-2243(81)90001-0.
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Expression of pregnancy specific beta 1-glycoprotein gene in human placenta and hydatiform mole.妊娠特异性β1-糖蛋白基因在人胎盘及葡萄胎中的表达
Biochem Int. 1989 May;18(5):999-1008.
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Pregnancy-specific beta 1-glycoprotein (SP1). Purification and partial characterization.妊娠特异性β1-糖蛋白(SP1)。纯化及部分特性鉴定。
Oncodev Biol Med. 1980;1(2):113-22.
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Pregnancy-specific beta 1 glycoprotein in rat: tissue distribution of the mRNA and identification of testicular cDNA clones.大鼠妊娠特异性β1糖蛋白:mRNA的组织分布及睾丸cDNA克隆的鉴定
Hum Reprod. 1988 Jul;3(5):687-92. doi: 10.1093/oxfordjournals.humrep.a136767.
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Processing of SP1 precursor in a cell-free system from poly(A+) mRNA of human placenta.在源自人胎盘多聚腺苷酸(poly(A+))mRNA的无细胞系统中对SP1前体进行加工。
Mol Biol Rep. 1988;13(1):45-51. doi: 10.1007/BF00805638.

引用本文的文献

1
Pregnancy-specific beta 1-glycoprotein: cDNA cloning, tissue expression, and species specificity of one member of the PS beta G family.
Hum Genet. 1989 Jun;82(3):239-43. doi: 10.1007/BF00291162.

本文引用的文献

1
Pregnancy-specific beta 1-glycoprotein as a screening test for at-risk pregnancies.妊娠特异性β1-糖蛋白作为高危妊娠的筛查试验。
Am J Obstet Gynecol. 1981 Nov 1;141(5):499-502. doi: 10.1016/s0002-9378(15)33268-3.
2
Efficient translation of tobacco mosaic virus RNA and rabbit globin 9S RNA in a cell-free system from commercial wheat germ.在由市售小麦胚芽制备的无细胞体系中烟草花叶病毒RNA和兔珠蛋白9S RNA的高效翻译。
Proc Natl Acad Sci U S A. 1973 Aug;70(8):2330-4. doi: 10.1073/pnas.70.8.2330.
3
Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A.
用葡萄球菌蛋白A-抗体吸附剂从细胞中快速分离抗原:抗体-抗原复合物与蛋白A相互作用的参数
J Immunol. 1975 Dec;115(6):1617-24.
4
Pregnancy specific beta1-glycoprotein--a product of the syncytiotrophoblast.妊娠特异性β1-糖蛋白——一种合体滋养层细胞的产物。
Experientia. 1976 Sep 15;32(9):1197. doi: 10.1007/BF01927624.
5
Plasma levels of pregnancy-specific beta 1-glycoprotein in complicated pregnancies.
Br J Obstet Gynaecol. 1977 Apr;84(4):258-63. doi: 10.1111/j.1471-0528.1977.tb12573.x.
6
Synthesis of human placental lactogen and human chorionic gonadotropin by polyribosomes and messenger RNA's from early and full term placentas.利用来自早期和足月胎盘的多核糖体及信使核糖核酸合成人胎盘催乳素和人绒毛膜促性腺激素。
J Biol Chem. 1976 May 25;251(10):2945-51.
7
Identification of mRNAs encoding the alpha and beta subunits of human choriogonadotropin.
J Biol Chem. 1978 Oct 10;253(19):7109-14.
8
Ribonuclease inhibitor from human placenta. Purification and properties.人胎盘核糖核酸酶抑制剂。纯化及性质
J Biol Chem. 1977 Aug 25;252(16):5904-10.
9
Isolation, cloning and sequence analysis of the cDNA for the alpha-subunit of human chorionic gonadotropin.
Nature. 1979 Oct 4;281(5730):351-6. doi: 10.1038/281351a0.
10
The compartmental distribution of oestrogens and pregnancy specific beta1 glycoprotein.雌激素和妊娠特异性β1糖蛋白的区室分布。
Br J Obstet Gynaecol. 1979 Feb;86(2):119-24. doi: 10.1111/j.1471-0528.1979.tb10578.x.