Koh S H, Cauchi M N
Eur J Obstet Gynecol Reprod Biol. 1981 Feb;11(4):215-9. doi: 10.1016/0028-2243(81)90001-0.
Using indirect immunofluorescence, beta 1-specific pregnancy glycoprotein (SP1) was localised to the amnion cells, as well as to trophoblastic cells. Cultures of fragments of amnion incubated with 3H-leucine for up to 72 h demonstrated that protein was synthesized by the amnion. Using antisera specific to SP1 it was shown that after 72 h incubation about 50% of the total protein could be precipitated. These studies demonstrate that the amnion cells are actively involved in the synthesis of SP1 and could account for the high level of this glycoprotein in amniotic fluid.
采用间接免疫荧光法，β1特异性妊娠糖蛋白（SP1）定位于羊膜细胞以及滋养层细胞。用3H-亮氨酸孵育羊膜碎片长达72小时的培养实验表明，羊膜能够合成蛋白质。使用针对SP1的抗血清显示，孵育72小时后，约50%的总蛋白可被沉淀。这些研究表明，羊膜细胞积极参与SP1的合成，这可以解释羊水中这种糖蛋白的高水平。
