一种热聚乙二醇法作为血浆分离的替代方法。

A heat polyethylene glycol procedure as an alternative method for plasmafractionation.

作者信息

Guddat W, Hillger K

出版信息

Folia Haematol Int Mag Klin Morphol Blutforsch. 1982;109(6):849-55.

Abstract

Polyethylenglycol 4000 was used in a combined process of fractionation at normal temperature for preparing gammaglobulin and albumin in a heat denaturation process. Albumin can be gained from human plasma with a high yield and purity by using a heat procedure at the presence of polyethylglycol 4000 and sodium octanate at 75 degrees C. The concentration of polyethylglycol 4000 varies during this heat procedures from 3-10% W/V.

摘要

聚乙二醇4000用于常温分级分离与热变性过程相结合的工艺，以制备丙种球蛋白和白蛋白。在75摄氏度下，通过在聚乙二醇4000和辛酸钠存在的情况下采用加热程序，可以从人血浆中高产量、高纯度地获得白蛋白。在该加热过程中，聚乙二醇4000的浓度在3-10%（W/V）之间变化。