一种热聚乙二醇法作为血浆分离的替代方法。
A heat polyethylene glycol procedure as an alternative method for plasmafractionation.
作者信息
Guddat W, Hillger K
出版信息
Folia Haematol Int Mag Klin Morphol Blutforsch. 1982;109(6):849-55.
PMID:6187637
Abstract
Polyethylenglycol 4000 was used in a combined process of fractionation at normal temperature for preparing gammaglobulin and albumin in a heat denaturation process. Albumin can be gained from human plasma with a high yield and purity by using a heat procedure at the presence of polyethylglycol 4000 and sodium octanate at 75 degrees C. The concentration of polyethylglycol 4000 varies during this heat procedures from 3-10% W/V.
摘要
聚乙二醇4000用于常温分级分离与热变性过程相结合的工艺，以制备丙种球蛋白和白蛋白。在75摄氏度下，通过在聚乙二醇4000和辛酸钠存在的情况下采用加热程序，可以从人血浆中高产量、高纯度地获得白蛋白。在该加热过程中，聚乙二醇4000的浓度在3-10%（W/V）之间变化。
相似文献
1
A heat polyethylene glycol procedure as an alternative method for plasmafractionation.一种热聚乙二醇法作为血浆分离的替代方法。
Folia Haematol Int Mag Klin Morphol Blutforsch. 1982;109(6):849-55.
2
An alternative method of large scale plasma fractionation for the isolation of serum albumin.
Blut. 1975 Feb;30(2):121-34. doi: 10.1007/BF01633966.
3
Heat-ethanol isolated serum albumin: discussion.热乙醇分离血清白蛋白：讨论
Folia Haematol Int Mag Klin Morphol Blutforsch. 1977;104(1):116-22.
4
Application of central composite design to the optimisation of aqueous two-phase extraction of human antibodies.中心复合设计在人源抗体双水相萃取优化中的应用。
J Chromatogr A. 2007 Feb 2;1141(1):50-60. doi: 10.1016/j.chroma.2006.11.075. Epub 2006 Dec 28.
5
A new high quality albumin for therapeutic use.一种新型的用于治疗的高质量白蛋白。
Dev Biol Stand. 1980;48:105-12.
6
Fractionation of outdated freeze-dried plasma: a comparative study of cold- and heat-ethanol fractionation procedures.过期冻干血浆的分馏：冷乙醇分馏法与热乙醇分馏法的对比研究
Folia Haematol Int Mag Klin Morphol Blutforsch. 1979;106(1):109-16.
7
Purification of IgG and albumin from human plasma by aqueous two phase system fractionation.用双水相体系分级分离法从人血浆中纯化 IgG 和白蛋白。
Biotechnol Prog. 2012 Jul;28(4):1005-11. doi: 10.1002/btpr.1565. Epub 2012 Jun 22.
8
Fractionation of plasma proteins with PEG.用聚乙二醇对血浆蛋白进行分级分离。
Haematologia (Budap). 1981;14(2):199-206.
9
Preparation of albumin from human plasma by heat denaturation method in plasma bag.在血浆袋中采用热变性法从人血浆中制备白蛋白。
Transfus Med. 2012 Dec;22(6):440-5. doi: 10.1111/j.1365-3148.2012.01188.x. Epub 2012 Oct 29.
10
Plasma fractionation: production and properties of albumin.血浆分离：白蛋白的生产与特性
Ann Immunol Hung. 1979;19:149-61.
Zotero 插件