Hølund B, Clemmensen I
Histochemistry. 1982;76(4):517-25. doi: 10.1007/BF00489906.
The influence of testicular hyaluronidase treatment on the immunohistochemical localization of fibronectin in different tissues (human articular cartilage, large intestine, synovial membrane and experimental granulation tissue) as well on frozen as on formaldehyde fixed, paraffin embedded tissue, has been studied using the indirect immunoperoxidase technique. Pretreatment with hyaluronidase is essential in demonstrating fibronectin in frozen sections of human articular cartilage. In the other tissues examined treatment with hyaluronidase was not essential, but gave a more optimal staining quality. The effect of hyaluronidase treatment was to some extent dependent on the duration of treatment. In formaldehyde fixed, paraffin processed tissue the improvement with hyaluronidase treatment was only seen when the hyaluronidase followed pepsin digestion of the deparaffinized tissue sections.
采用间接免疫过氧化物酶技术，研究了睾丸透明质酸酶处理对不同组织（人关节软骨、大肠、滑膜和实验性肉芽组织）中纤连蛋白免疫组化定位的影响，这些组织包括冷冻组织以及经甲醛固定、石蜡包埋的组织。在用透明质酸酶预处理后，才能在人关节软骨的冷冻切片中显示出纤连蛋白。在其他检测的组织中，透明质酸酶处理并非必需，但能使染色质量更佳。透明质酸酶处理的效果在一定程度上取决于处理时间。在经甲醛固定、石蜡处理的组织中，只有当透明质酸酶在对脱石蜡组织切片进行胃蛋白酶消化之后使用时，才能看到其处理带来的改善效果。
