Lerch P G, van de Rijn M, Schrier P, Terhorst C
Hum Immunol. 1983 Jan;6(1):13-30. doi: 10.1016/0198-8859(83)90070-8.
The human thymic differentiation antigen T6, which was found to be associated with beta 2-microglobulin, was compared to the HLA-A,B antigens. Using a heteroantiserum prepared against denatured heavy chains of HLA-A,B antigens, no cross-reactivity with denatured T6 could be detected. The molecular weight of the protein backbone of T6 was found to be 34,000 as compared to 40,000 for the HLA-A,B antigens. Also, not only was the percentage of carbohydrate of T6 (25-35%) different from the HLA-A,B antigens (10%), but lectin binding studies showed that their sugar composition may differ. The two forms of T6, which previously had been found on MOLT-4 cells, appeared to have different levels of glycosylation, but apparently had the same protein backbone. T6, like HLA, has a hydrophobic domain, since it could be labeled with [125I]iodonaphthylazide. We conclude from these studies that T6 may be a class I MHC antigen which is different from the classical HLA-A,B antigens.
已发现与人β2-微球蛋白相关的人类胸腺分化抗原T6与HLA-A、B抗原进行了比较。使用针对HLA-A、B抗原变性重链制备的异种抗血清，未检测到与变性T6的交叉反应。发现T6蛋白质主链的分子量为34,000，而HLA-A、B抗原为40,000。此外，不仅T6的碳水化合物百分比（25-35%）与HLA-A、B抗原（10%）不同，而且凝集素结合研究表明它们的糖组成可能不同。先前在MOLT-4细胞上发现的两种形式的T6似乎具有不同程度的糖基化，但显然具有相同的蛋白质主链。T6与HLA一样，具有一个疏水区，因为它可以用[125I]碘萘叠氮化物标记。我们从这些研究中得出结论，T6可能是一种与经典HLA-A、B抗原不同的I类MHC抗原。
