Terhorst C, van Agthoven A, LeClair K, Snow P, Reinherz E, Schlossman S
Cell. 1981 Mar;23(3):771-80. doi: 10.1016/0092-8674(81)90441-4.
Three human thymic cell-surface antigens T6, T9 and T10, previously defined by monoclonal antibodies, were analyzed using immunoprecipitation techniques. The antigen T6 was found to be a 49,000 dalton glycoprotein, which is associated with beta 2-microglobulin, the small subunit (12,000 daltons) of the HLA-A, -B, and -C antigens. The target antigen for the monoclonal reagent anti-T9 was found to be a glycoprotein of 94,000 daltons, which appears as a disulfide-linked dimer of 190,000 daltons on the cell surface. The antigen precipitated by the anti-T10 antibody is a 45,000 dalton glycoprotein. We present preliminary evidence that all three cell-surface proteins may be integral membrane proteins. These findings, in addition to the distribution patterns, suggest that the T6 antigen is the human homolog of the murine thymus leukemia (TL) antigen.
利用免疫沉淀技术分析了先前由单克隆抗体定义的三种人类胸腺细胞表面抗原T6、T9和T10。发现抗原T6是一种49000道尔顿的糖蛋白，它与β2-微球蛋白相关，β2-微球蛋白是HLA-A、-B和-C抗原的小亚基（12000道尔顿）。发现单克隆试剂抗-T9的靶抗原是一种94000道尔顿的糖蛋白，在细胞表面它表现为190000道尔顿的二硫键连接的二聚体。抗-T10抗体沉淀的抗原是一种45000道尔顿的糖蛋白。我们提供了初步证据表明这三种细胞表面蛋白可能都是整合膜蛋白。这些发现，除了分布模式外，还表明T6抗原是小鼠胸腺白血病（TL）抗原的人类同源物。
