Eardley D D
J Immunol. 1983 May;130(5):2209-13.
Clones of sheep erythrocyte-(SRBC) specific helper T cells with the surface phenotype Thy-1+, Ly-1+, Ly-2- have been derived that grow in vitro in the absence of exogenous antigen or added growth factors. The IL 2-independent clone, 101.6 has been shown to produce a supernatant factor that augments the primary anti-SRBC but not anti-burro RBC responses of whole spleen cells or Ly-1 T plus B cell cultures. The supernatant does not help B cells directly. This augmenting activity is terminated "co-helper" because the enhancement requires the presence of normal Ly-1 T cells. The supernatant of 101.6 was not shown to contain IL 2; co-helper activity was distinguishable from IL 2 activity by absorption with SRBC but not with Con A blasts, and we observed that co-helper activity does not act on spleen cells that differ at the major histocompatibility complex.
已获得具有表面表型Thy-1+、Ly-1+、Ly-2-的绵羊红细胞（SRBC）特异性辅助性T细胞克隆，这些克隆在无外源性抗原或添加生长因子的情况下可在体外生长。不依赖白细胞介素2（IL-2）的克隆101.6已被证明能产生一种上清因子，该因子可增强全脾细胞或Ly-1 T加B细胞培养物对SRBC的初次应答，但不能增强对驴红细胞的应答。该上清液不能直接作用于B细胞。这种增强活性被称为“协同辅助”，因为这种增强需要正常Ly-1 T细胞的存在。101.6的上清液未显示含有IL-2；协同辅助活性通过用SRBC而非伴刀豆球蛋白A（Con A）母细胞吸附可与IL-2活性区分开来，并且我们观察到协同辅助活性对主要组织相容性复合体不同的脾细胞不起作用。
