Basch R S, Berman J W, Lakow E
J Immunol Methods. 1983 Feb 11;56(3):269-80. doi: 10.1016/s0022-1759(83)80016-7.
Positive immunoselection is the direct selection and recovery of cells which express a given specificity from among a heterogeneous group of contaminating cells. A variety of methods are available to effect such separations. The principles of affinity chromatography, using solid-phase matrices or cellular immunoadsorbents, are extensively used. Liquid-phase positive immunoselection can also be performed using either a fluorescence-activated cell sorter or by using 'cellular engineering' to protect a cell from an otherwise noxious environment. The enzyme catalase coupled to specific antibody has been used for this purpose and renders cells resistant to hydrogen peroxide. The various positive immunoselection techniques available are reviewed and evaluated in the following report.
阳性免疫选择是从一群异质性污染细胞中直接选择并回收表达特定特异性的细胞。有多种方法可用于实现这种分离。利用固相基质或细胞免疫吸附剂的亲和层析原理被广泛应用。液相阳性免疫选择也可使用荧光激活细胞分选仪或通过“细胞工程”来保护细胞免受有害环境影响的方式进行。与特异性抗体偶联的过氧化氢酶已用于此目的，可使细胞对过氧化氢产生抗性。以下报告对现有的各种阳性免疫选择技术进行了综述和评估。
