Dussaix E, Gregoire A, Chany C, Thang D C, Thang M N
J Gen Virol. 1983 Feb;64 (Pt 2):285-90. doi: 10.1099/0022-1317-64-2-285.
Mouse interferon (IFN) was produced to high titres after induction of Ehrlich ascites tumour cells with Sendai virus by using an improved procedure. The IFN molecules were labelled during their synthesis by the incorporation of [3H]leucine and [3H]lysine. Electrophoretically homogeneous labelled IFN with a molecular weight of 34000 was obtained after a two-step purification procedure using poly(I)-agarose and octyl-Sepharose column chromatography followed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. The specific radioactivity of this IFN was about 10 ct/min/IFN unit.
采用改进的方法，用仙台病毒诱导艾氏腹水瘤细胞后，可产生高滴度的小鼠干扰素（IFN）。在干扰素分子合成过程中，通过掺入[3H]亮氨酸和[3H]赖氨酸进行标记。经过两步纯化程序，先用聚（I）-琼脂糖和辛基-琼脂糖柱层析，再用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳，得到了分子量为34000的电泳纯标记干扰素。这种干扰素的比放射性约为10次计数/分钟/干扰素单位。
