Hirohashi S, Shimosato Y, Ino Y, Kishi K, Ohkura H, Mukojima T
Jpn J Clin Oncol. 1983 Mar;13(1):37-43.
The distribution of alpha-fetoprotein (AFP) and immunoreactive carcinoembryonic antigen (CEA) in 62 hepatocellular carcinomas (HCC) and five hepatoblastomas (HBL) all surgically removed was studied by an immunohistochemical method, and the results were compared with the levels of the antigens in the patients' serum. AFP was present as coarse granules in the cytoplasm of immature tumor cells. AFP-positive tumor cells were detected in 34/43 cases (79.1%) with serum AFP levels higher than 400 ng/ml and in 1/24 (4.2%) of the remaining cases. They were present in 8/32 (25%) cases of relatively well-differentiated HCC (Edmondson's grade I or II), 22/32 (68.8%) of relatively poorly differentiated HCC (Edmondson's grade III or IV), and 5/5 of HBL. An antigen immunoreactive with a conventional rabbit anti-CEA immunoglobulin (DAKO) but not with a murine monoclonal anti-CEA antibody (Hybritech) was present on the surface of bile canaliculi of both non-neoplastic and neoplastic hepatocytes. This CEA cross-reactive differentiation antigen was more often found in relatively well-differentiated tumors, and the presence of this antigen in tumors did not correlate with patients' serum CEA levels. In conclusion, CEA of an oncofetal nature was not produced by either HCC or HBL.
采用免疫组化方法研究了62例手术切除的肝细胞癌（HCC）和5例肝母细胞瘤（HBL）中α-甲胎蛋白（AFP）和免疫反应性癌胚抗原（CEA）的分布情况，并将结果与患者血清中的抗原水平进行了比较。AFP以粗颗粒形式存在于未成熟肿瘤细胞的细胞质中。血清AFP水平高于400 ng/ml的43例患者中有34例（79.1%）检测到AFP阳性肿瘤细胞，其余24例中有1例（4.2%）检测到。它们存在于8/32（25%）例分化相对良好的HCC（Edmondson分级I或II级）、22/32（68.8%）例分化相对较差的HCC（Edmondson分级III或IV级）以及5/5的HBL中。一种与传统兔抗CEA免疫球蛋白（DAKO）发生免疫反应但不与鼠单克隆抗CEA抗体（Hybritech）发生免疫反应的抗原存在于非肿瘤性和肿瘤性肝细胞的胆小管表面。这种CEA交叉反应性分化抗原在分化相对良好的肿瘤中更常见，肿瘤中该抗原的存在与患者血清CEA水平无关。总之，HCC和HBL均不产生具有肿瘤胎儿性质的CEA。
