Kinoyama S, Yamada G, Nagashima H
Gastroenterol Jpn. 1986 Apr;21(2):152-61.
The precise sites of alpha-fetoprotein (AFP) synthesis and ultrastructural features and differences of AFP-producing cells were observed in periodate-lysine-paraformaldehyde fixed, frozen liver tissues from four human hepatocellular carcinoma (HCC) patients and three human fetuses using the direct (horseradish peroxidase-labeled Fab' fraction of anti-human AFP) immunoperoxidase method. We demonstrated that AFP was located in the membrane and cisternae of rough endoplasmic reticulum, membrane-bound ribosomes, perinuclear space and Golgi apparatus. The location and intensity of immunoreaction products of AFP in hepatoma cells varied from cell to cell and case to case, while these features tended to be regular in fetal hepatocytes. We did not observe ultrastructural differences between AFP-producing and non-producing cells adjacent to each other. These observations indicate that AFP production does not occur in morphologically distinct cell populations of hepatoma tissue and that hepatoma tissue is functionally much more heterogeneous than fetal liver.
采用直接（抗人甲胎蛋白辣根过氧化物酶标记的Fab’片段）免疫过氧化物酶法，在4例人类肝细胞癌（HCC）患者和3例人类胎儿经高碘酸盐-赖氨酸-多聚甲醛固定的冷冻肝组织中，观察甲胎蛋白（AFP）的精确合成位点、AFP产生细胞的超微结构特征及差异。我们证实，AFP位于粗面内质网的膜和池、膜结合核糖体、核周间隙和高尔基体中。AFP免疫反应产物在肝癌细胞中的定位和强度因细胞和病例而异，而在胎儿肝细胞中这些特征趋于规则。我们未观察到彼此相邻的AFP产生细胞和非产生细胞之间的超微结构差异。这些观察结果表明，AFP并非在肝癌组织形态学上不同的细胞群体中产生，且肝癌组织在功能上比胎儿肝脏的异质性更强。
