Sweet W J, Peterson J A
J Bacteriol. 1978 Jan;133(1):217-24. doi: 10.1128/jb.133.1.217-224.1978.
Optical absorbance difference spectra of membrane vesicles prepared from aerobically grown Pseudomonas putida indicated that, when harvested in logarithmic phase, the cells contained one c-type cytochrome and two or three b-type cytochromes, one of which was cytochrome o. As the cells grew into stationary phase and the oxygen concentration of the medium dropped to essentially zero, an additional component believed to be cytochrome d was produced. Both the o- and d-type cytochromes might function as terminal oxidases. No a-type cytochromes could be detected at any stage of growth. Polarographic measurement of oxygen utilization revealed that cyanide and azide are effective inhibitors of the oxidation of ascorbate coupled with 2,6-dichlorophenolindophenol or N,N,N',N'-tetramethyl-p-phenylenediamine in respiratory particles from either log-phase or stationary-phase cells. Reduced nicotinamide adenine dinucleotide- or succinate-dependent oxygen utilization, however, was sensitive to these inhibitors only in log-phase particles. These results indicate that an alternate terminal oxidase may be synthesized by this organism in response to restricted oxygen availability and that branching of the respiratory system may result.
从需氧生长的恶臭假单胞菌制备的膜囊泡的光吸收差光谱表明，对数生长期收获的细胞含有一种c型细胞色素和两到三种b型细胞色素，其中一种是细胞色素o。随着细胞进入稳定期且培养基中的氧气浓度基本降至零，会产生另一种据信是细胞色素d的成分。o型和d型细胞色素都可能作为末端氧化酶发挥作用。在生长的任何阶段都检测不到a型细胞色素。对氧气利用的极谱测量表明，氰化物和叠氮化物是需氧生长的恶臭假单胞菌对数期或稳定期细胞呼吸颗粒中抗坏血酸与2,6 - 二氯酚靛酚或N,N,N',N'-四甲基对苯二胺偶联氧化的有效抑制剂。然而，还原型烟酰胺腺嘌呤二核苷酸或琥珀酸依赖性氧气利用仅在对数期颗粒中对这些抑制剂敏感。这些结果表明，这种生物体可能会因氧气供应受限而合成一种替代末端氧化酶，并且可能导致呼吸系统分支。
