凡氏甲烷球菌tRNA的组成与特性
Composition and Characterization of tRNA from Methanococcus vannielii.
作者信息
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出版信息
J Bacteriol. 1978 Jan;133(1):240-50. doi: 10.1128/jb.133.1.240-250.1978.
Abstract
Purified bulk tRNA from Methanococcus vanielii (carbon source, formate) showed variation in the modified nucleoside pattern reported for Escherichia coli as analyzed by both ion-exchange and thin-layer chromatography. Ribothymidine and 7-methylguanosine were absent; 1-methyladenosine, 1-methylguanosine, N2-methylguanosine, N2,N2-dimethylguanosine, thiolated nucleosides, pseudouridine, dihydrouridine, and O2'-methylcytidine were quantitated. In vitro methylation by M. Vannielii extracts with S-adenosylmethionine and undermethylated E. coli tRNA revealed active tRNA methyltransferases for formation of methylated residues found in native M. vannielii tRNA, but none for the formation of 7-methylguanosine or ribothymidine. The native M. vannielii tRNA became methylated in the 7-methylguanosine position by E. Coli extracts, but ribothymidine was not formed. Both M. vannielii and E. coli tRNA methyltransferases produced unidentified methylated residues in tRNA's lacking or deficient in ribothymidine.
摘要
对来自嗜甲烷球菌（碳源为甲酸）的纯化大量tRNA进行分析，通过离子交换色谱法和薄层色谱法分析发现，其修饰核苷模式与报道的大肠杆菌的模式存在差异。核糖胸腺嘧啶核苷和7-甲基鸟苷缺失；对1-甲基腺苷、1-甲基鸟苷、N2-甲基鸟苷、N2,N2-二甲基鸟苷、硫醇化核苷、假尿苷、二氢尿苷和2'-O-甲基胞苷进行了定量分析。用嗜甲烷球菌提取物和S-腺苷甲硫氨酸对未充分甲基化的大肠杆菌tRNA进行体外甲基化，结果显示存在活性tRNA甲基转移酶，可形成天然嗜甲烷球菌tRNA中发现的甲基化残基，但没有形成7-甲基鸟苷或核糖胸腺嘧啶核苷的活性。天然嗜甲烷球菌tRNA在7-甲基鸟苷位置可被大肠杆菌提取物甲基化，但不会形成核糖胸腺嘧啶核苷。嗜甲烷球菌和大肠杆菌的tRNA甲基转移酶在缺乏核糖胸腺嘧啶核苷或核糖胸腺嘧啶核苷含量不足的tRNA中都会产生未鉴定的甲基化残基。
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