Brown D T, Morris G F, Chodchoy N, Sprecher C, Marzluff W F
Nucleic Acids Res. 1985 Jan 25;13(2):537-56. doi: 10.1093/nar/13.2.537.
The genes coding for U1 RNA in the sea urchin L. variegatus are present in a 1400 base pair tandem repeat. One member of the repeat has been cloned and its sequence determined. The repeat unit contains a single copy of the gene for L. variegatus U1 RNA. This gene encodes an RNA which is 75% homologous to mammalian U1 RNA. The L. variegatus U1 RNA could assume a secondary structure similar to that proposed for other U1 RNAs. In addition the L. variegatus U1 RNA is precipitated by anti-SM and anti-RNP antisera. Analysis of the L. variegatus genomic DNA using the cloned U1 gene as a probe reveals a major and a minor type of repeat unit. The two repeated units are the same length but differ in a number of restriction enzyme sites clustered 200-500 bases down-stream from the gene. The monomer we have cloned and sequenced is a representative of the minor repeat. A sequence (GATAA) which is -41 to -37 bases 5' to the gene has homology to the putative RNA polymerase II promoter. Fifteen bases 3' of the gene is a sequence (CAAAGAAAGAAAA) which is very similar to the sequence found 3' of the sea urchin histone genes. The two Hha I, Hpa II and Ava I sites in the repeat are all unmethylated in sperm DNA.
在多色紫球海胆中，编码U1 RNA的基因存在于一个1400碱基对的串联重复序列中。该重复序列的一个成员已被克隆并测定了其序列。重复单元包含一个多色紫球海胆U1 RNA基因的单拷贝。该基因编码一种与哺乳动物U1 RNA有75%同源性的RNA。多色紫球海胆U1 RNA可呈现出与其他U1 RNA所推测的二级结构相似的结构。此外，多色紫球海胆U1 RNA能被抗SM和抗RNP抗血清沉淀。用克隆的U1基因作为探针分析多色紫球海胆基因组DNA，揭示出一种主要的和一种次要的重复单元类型。这两种重复单元长度相同，但在基因下游200 - 500个碱基处聚集的多个限制性酶切位点存在差异。我们已克隆并测序的单体是次要重复序列的代表。在基因5'端 - 41至 - 37个碱基处的一个序列（GATAA）与推定的RNA聚合酶II启动子具有同源性。基因3'端的15个碱基是一个序列（CAAAGAAAGAAAA），它与在海胆组蛋白基因3'端发现的序列非常相似。重复序列中的两个Hha I、Hpa II和Ava I位点在精子DNA中均未甲基化。
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