Doornbos J, Wreesmann C T, Van Boom J H, Altona C
Eur J Biochem. 1983 Apr 5;131(3):571-9. doi: 10.1111/j.1432-1033.1983.tb07301.x.
Proton NMR studies at 300 MHz and 500 MHz are reported on the ribotetranucleotide A-A-C-C. The complete 1H-NMR spectral assignment at 20 degrees C is given. Two-dimensional NMR was used to elucidate spin multiplets in 'crowded' regions. Nuclear Overhauser enhancement (NOE) experiments made an unambiguous spectral assignment possible and yielded information on interproton distances. The N/S equilibrium of the riboses and the rotamer populations around some backbone torsion angles are presented. A large preference for N-type ribose and gamma+ and beta t backbone torsions is observed, in particular in the central A-C unit of A-A-C-C. Information on distances between protons of different nucleotide units, obtained from NOE experiments, constitutes a probe of base-base stacking. It is concluded that A-A-C-C offers a good model for RNA single-strand conformation.
报告了在300兆赫和500兆赫下对核糖四核苷酸A - A - C - C进行的质子核磁共振研究。给出了在20摄氏度时完整的1H - NMR光谱归属。二维核磁共振用于阐明“拥挤”区域中的自旋多重峰。核Overhauser增强（NOE）实验使得明确的光谱归属成为可能，并产生了质子间距离的信息。展示了核糖的N/S平衡以及一些主链扭转角周围的旋转异构体群体。观察到对N型核糖以及γ+和β t主链扭转有很大的偏好，特别是在A - A - C - C的中央A - C单元中。从NOE实验获得的不同核苷酸单元质子间距离的信息构成了碱基堆积的一个探针。得出结论，A - A - C - C为RNA单链构象提供了一个良好的模型。
