Traill K N, Böck G, Boyd R, Wick G
Dev Comp Immunol. 1983 Winter;7(1):111-25. doi: 10.1016/0145-305x(83)90060-5.
Different procedures for enrichment of peripheral blood leukocytes (PBL) from whole chicken blood have been compared in terms of the percentage yield of lymphocytes and thrombocytes. The yield of thrombocytes was low in the buffy coat cells of heparinised blood, but high in PBL enriched from heparinised blood over a Ficoll-Paque density gradient and in PBL prepared from citrated blood by either technique. The fluorescence activated cell sorter (FACS) has been used to separate thrombocytes from lymphocytes in PBL by sorting of the negative cells after staining with specific anti-T and anti-B cell sera (or anti-immunoglobulin). The sorted cells were more than 99% viable, more than 98% thrombocytes by antigenic and morphological criteria and did not respond in vitro to T and B cell mitogens. Absorption studies on a turkey anti-bursa cell serum (ABST) which is cross-reactive for B cells and thrombocytes (8) indicated that the cross-reactivity is attributable to two (or more) antibody specificities in the serum rather than to one antibody directed at shared determinants on the two cell types.
已对从全鸡血液中富集外周血白细胞（PBL）的不同程序在淋巴细胞和血小板的回收率方面进行了比较。在肝素化血液的血沉棕黄层细胞中血小板回收率较低，但通过Ficoll-Paque密度梯度从肝素化血液中富集的PBL以及用两种技术之一从枸橼酸盐血液制备的PBL中血小板回收率较高。荧光激活细胞分选仪（FACS）已用于通过用特异性抗T和抗B细胞血清（或抗免疫球蛋白）染色后对阴性细胞进行分选，从PBL中的淋巴细胞中分离血小板。分选的细胞活力超过99%，根据抗原和形态学标准，血小板超过98%，并且在体外对T和B细胞有丝分裂原无反应。对火鸡抗法氏囊细胞血清（ABST）的吸收研究表明，该血清对B细胞和血小板具有交叉反应性（8），交叉反应性归因于血清中的两种（或更多）抗体特异性，而不是针对两种细胞类型上共享决定簇的一种抗体。
