Hála K, Boyd R L, Wolf H, Böck G, Wick G
Scand J Immunol. 1984 Jul;20(1):15-9. doi: 10.1111/j.1365-3083.1984.tb00972.x.
The functional of B-L (Ia-equivalent)-positive (B-L+) adn -negative (B-L-) chicken peripheral blood lymphocytes (PBL) was studied in vitro and in vivo. The PBL were first stained in direct immunofluorescence tests with a fluorescein isothiocyanate-labelled anti-B-L alloantiserum and then separated by means of a fluorescence-activated cell sorter. In agreement with our previous findings, B-L- cells showed functional properties of T lymphocytes, responding to concanavalin A and phytohaemagglutinin-P in vitro and inducing a graft-versus-host (GVH) reaction when injected into allogeneic embryos. Sorted B-L+ gave no responses in any of these assays. Neither B-L+ nor B-L- cells, when tested alone, responded significantly to pokeweed mitogen, but mixtures of the two restored the responsiveness to that of the original unsorted suspension. Of the B-L+ PBL, 10% were T cells, which may account for the low GVH reactivity given by this population.
对鸡的B-L（Ia等效）阳性（B-L+）和阴性（B-L-）外周血淋巴细胞（PBL）的功能进行了体内和体外研究。首先用异硫氰酸荧光素标记的抗B-L同种异体抗血清对PBL进行直接免疫荧光试验染色，然后通过荧光激活细胞分选仪进行分离。与我们之前的研究结果一致，B-L-细胞表现出T淋巴细胞的功能特性，在体外对刀豆球蛋白A和植物血凝素-P有反应，并且当注入同种异体胚胎时会引发移植物抗宿主（GVH）反应。分选后的B-L+细胞在任何这些试验中均无反应。单独测试时，B-L+和B-L-细胞对商陆有丝分裂原均无明显反应，但两者的混合物可恢复到原始未分选悬浮液的反应性。在B-L+ PBL中，10%是T细胞，这可能是该群体GVH反应性较低的原因。
