[Cultured lymphocytes in chronic alcoholics].

Lymphocytes were isolated from the peripheral blood of 80 chronic alcoholics without an evident liver damage and filled up with 4 different mitogens. The DNA synthesis was investigated by means of the 3H thymidine incorporation rate. They were referred to a group of 15 healthy test persons. An increased proliferation rate of B-lymphocytes could be demonstrated, whereas there was no evidence for a change in the stimulatibility of T-lymphocytes. It was only the direct impact of ethyl alcohol on lymphocytes that produced a significant decrease in the ability of T-cell reactivity, whereas a further increase of stimulatibility could be observed in B-cells. Our findings allow the conclusion to be drawn that an immediate toxic impact of ethanol on lymphocytes can be identified in chronic alcoholics without manifest liver damage.