Schwager J, Schulze J
Centre de Recherche en Nutrition Animale, Socìeté Chimique Roche, Village-Neuf, France.
Int J Vitam Nutr Res. 1997;67(1):10-6.
The influence of ascorbic acid (AA) on lymphocyte functions was examined in vitro and ex vivo in peripheral blood mononuclear cells (PBMC) of vitamin C-deficient pigs, which are unable to synthesise ascorbic acid. AA is accumulated to physiological levels in PBMC in vitro. The cell proliferation induced by T lymphocyte mitogens was unaltered at all AA concentrations tested (0-400 micrograms/ml, i.e., 0-2.3 mM). Conversely, the response to pokeweed mitogen (PWM) which activates T and B lymphocytes was significantly reduced with increasing intracellular and extracellular AA concentrations. The response to lipopolysaccharide (LPS) showed a tendency to increase at low (9 microM) and was significantly reduced at high AA concentrations (> 36 microM). The IL2 production induced by PWM (but not by concanavalin A (Con A) or phytohemagglutinin (PHA)) decreased at high AA (> 142 microM). In contrast, IL6 production induced by mitogens was not dependent on AA concentrations. In concordance with these results, AA-depleted PBMC which were obtained from pigs that were fed an AA-free diet, displayed an increasing response to LPS and PWM. Collectively, the data indicate that ascorbic acid selectively influences the proliferation of B lymphocytes and negatively acts on IL2 production by T lymphocytes when a threshold of saturation is exceeded.
在体外和体内研究了抗坏血酸（AA）对维生素C缺乏猪外周血单个核细胞（PBMC）淋巴细胞功能的影响，这些猪无法合成抗坏血酸。在体外，PBMC中的AA积累到生理水平。在所有测试的AA浓度（0-400微克/毫升，即0-2.3毫摩尔）下，T淋巴细胞有丝分裂原诱导的细胞增殖未发生改变。相反，随着细胞内和细胞外AA浓度的增加，对激活T和B淋巴细胞的商陆有丝分裂原（PWM）的反应显著降低。对脂多糖（LPS）的反应在低浓度（9微摩尔）时有增加的趋势，在高AA浓度（>36微摩尔）时显著降低。在高AA（>142微摩尔）时，PWM诱导的IL2产生（但不是伴刀豆球蛋白A（Con A）或植物血凝素（PHA）诱导的）降低。相反，有丝分裂原诱导的IL6产生不依赖于AA浓度。与这些结果一致，从喂食无AA饮食的猪获得的AA耗尽的PBMC对LPS和PWM的反应增加。总体而言，数据表明，当超过饱和阈值时，抗坏血酸选择性地影响B淋巴细胞的增殖，并对T淋巴细胞产生的IL2产生负面影响。
