一种细胞毒性单克隆IgM抗体（Tü 101），它针对HLA - A同种特异性A2和A28之间共有的一个抗原决定簇。

A cytotoxic monoclonal IgM antibody (Tü 101) directed against an antigenic determinant shared between the HLA-A allospecificities A2 and A28.

作者信息

Müller C, Liangru S, Schneider M, Ziegler A, Wernet P

出版信息

Hum Immunol. 1983 Apr;6(4):189-97. doi: 10.1016/0198-8859(83)90092-7.

Abstract

A complement-fixing murine monoclonal IgM antibody (TU 101) strictly directed against a supertypic determinant present on the HLA-A locus antigens A2 and A28 was defined from a fusion experiment employing T cell blasts as immunizing cells. The specificity of this antibody was established in the microcytotoxicity assay on 91 normal Caucasian blood donors, as well as in the SpA-Ig assay on a panel of lympho- and hematopoietic cell lines. TU 101 segregates only with its defined HLA allotypes in families. This reagent may be of particular value as a probe for analyzing a molecular relationship of different antigenic determinants on the HLA-A2 and A28 specificities in comparison with two recently defined anti-HLA-A2/A28 monoclonal antibodies and may help to characterize structural variations of these HLA-molecules on a serological and immunochemical basis.

摘要

通过以T细胞母细胞作为免疫细胞的融合实验，鉴定出一种严格针对HLA - A位点抗原A2和A28上存在的超型决定簇的补体结合鼠单克隆IgM抗体（TU 101）。该抗体的特异性在对91名正常高加索献血者的微量细胞毒性试验中得到确定，以及在一组淋巴细胞和造血细胞系的葡萄球菌A蛋白 - Ig试验中得到确定。在家族中，TU 101仅与其确定的HLA同种异型分离。与最近定义的两种抗HLA - A2/A28单克隆抗体相比，该试剂作为一种探针，对于分析HLA - A2和A28特异性上不同抗原决定簇的分子关系可能具有特殊价值，并且可能有助于在血清学和免疫化学基础上表征这些HLA分子的结构变异。