Voisin P, Larcan A, Stoltz J F
J Mal Vasc. 1983;8(1):33-6.
A Couette type coaxial cylinder viscosimeter was used to evaluate the influence of deformation rates up to 20,000/s on blood platelets, in the presence or absence of red cells. Plasma lactate dehydrogenase and haemoglobin levels were determined in order to assess cell lysis and the estimation of beta-thromboglobulin (beta-TG) was considered to be an index of platelet activation. Results for whole blood revealed a change in red cells from 10,000/s upwards, which increased as the rate rose. At the same time, beta-TG rose slowly from the lowest deformation rates then considerably, in relation with the liberation of erythrocytic substances. The rise in LDH and beta-TG levels in platelet rich plasma (PRP) remained small up to 7,000/s approximately, then increased very rapidly, suggesting earlier mechanical and biochemical activation than in whole blood.
使用库埃特型同轴圆筒粘度计评估高达20000/秒的变形率对有无红细胞存在时血小板的影响。测定血浆乳酸脱氢酶和血红蛋白水平以评估细胞裂解，β-血小板球蛋白（β-TG）的测定被视为血小板活化的指标。全血结果显示，从10000/秒以上红细胞发生变化，且随着速率升高而增加。与此同时，β-TG从最低变形率开始缓慢上升，然后随着红细胞物质的释放而大幅上升。富含血小板血浆（PRP）中LDH和β-TG水平的升高在约7000/秒之前一直较小，然后非常迅速地增加，表明比全血更早发生机械和生化活化。
