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网格蛋白三聚体中轻链的定位与分布。

Location and distribution of the light chains in clathrin trimers.

作者信息

Kirchhausen T, Harrison S C, Parham P, Brodsky F M

出版信息

Proc Natl Acad Sci U S A. 1983 May;80(9):2481-5. doi: 10.1073/pnas.80.9.2481.

DOI:10.1073/pnas.80.9.2481
PMID:6189124
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC393849/
Abstract

Mouse monoclonal antibodies have been made that are specific for one of the light chains (LC-A) of calf brain clathrin. The determinant they recognize has been mapped by immunoelectron microscopy and shown to lie near the center of a clathrin trimer. Quantitative immunoprecipitation experiments with one of the monoclonal antibodies suggests that, despite the overall 1:2 stoichiometry, the light chain composition of an individual clathrin trimer is nearly random.

摘要

已经制备出针对小牛脑网格蛋白轻链之一（LC - A）的小鼠单克隆抗体。通过免疫电子显微镜对它们所识别的决定簇进行了定位，结果表明该决定簇位于网格蛋白三聚体的中心附近。用其中一种单克隆抗体进行的定量免疫沉淀实验表明，尽管总体化学计量比为1:2，但单个网格蛋白三聚体的轻链组成几乎是随机的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8e5/393849/cc5e6c9d326f/pnas00635-0076-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8e5/393849/2ede802a64c3/pnas00635-0073-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8e5/393849/41ac14d8e4df/pnas00635-0074-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8e5/393849/8e78523127e8/pnas00635-0074-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8e5/393849/66050065bed5/pnas00635-0075-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8e5/393849/2e8061006681/pnas00635-0075-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8e5/393849/25118605fe90/pnas00635-0075-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8e5/393849/4f4f761ea848/pnas00635-0075-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8e5/393849/cc5e6c9d326f/pnas00635-0076-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8e5/393849/2ede802a64c3/pnas00635-0073-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8e5/393849/41ac14d8e4df/pnas00635-0074-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8e5/393849/8e78523127e8/pnas00635-0074-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8e5/393849/66050065bed5/pnas00635-0075-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8e5/393849/2e8061006681/pnas00635-0075-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8e5/393849/25118605fe90/pnas00635-0075-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8e5/393849/4f4f761ea848/pnas00635-0075-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8e5/393849/cc5e6c9d326f/pnas00635-0076-a.jpg

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Clathrin Light Chains: Not to Be Taken so Lightly.网格蛋白轻链：不可小觑
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本文引用的文献

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Assembly units of clathrin coats.网格蛋白包被的组装单位。
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Assembly and packing of clathrin into coats.网格蛋白组装并包装成衣被。
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Taming the Triskelion: Bacterial Manipulation of Clathrin.驯服三联体：细菌对网格蛋白的操纵。
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Hsc70-induced changes in clathrin-auxilin cage structure suggest a role for clathrin light chains in cage disassembly.网格蛋白辅助蛋白笼结构的热休克蛋白 70 诱导变化表明网格蛋白轻链在笼解体中的作用。
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Single-molecule analysis of a molecular disassemblase reveals the mechanism of Hsc70-driven clathrin uncoating.单分子分析揭示了 Hsc70 驱动网格蛋白解包的机制
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