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[流感病毒神经氨酸酶抗原特异性的测定]

[Determination of the antigenic specificity of the neuraminidase of influenza viruses].

作者信息

Molibog E V, Selivanov Ia M, Mel'nichenko E I, Isachenko V A, Zakstel'skaia L Ia

出版信息

Vopr Virusol. 1983 Jan-Feb;28(1):46-50.

PMID:6189296
Abstract

The principal possibility of using beta-propiolactone-inactivated and lyophilized antigens of influenza A and B viruses and of mouse immune ascitic fluids to them for the determination of the antigenic specificity of neuraminidase has first been established. A simpler and easier method requiring no expensive reagents: inhibition of influenza virus release from erythrocytes was tested. This method is as specific as the labour-consuming and difficult test of neuraminidase activity inhibition, and allows a rapid and accurate identification of the type of neuraminidase of influenza viruses of various origins to be made.

摘要

首次证实了使用经β-丙内酯灭活并冻干的甲型和乙型流感病毒抗原以及针对这些抗原的小鼠免疫腹水来测定神经氨酸酶抗原特异性的主要可能性。测试了一种更简单、更易操作且无需昂贵试剂的方法:抑制流感病毒从红细胞中释放。该方法与耗时且困难的神经氨酸酶活性抑制试验一样具有特异性,并且能够快速、准确地鉴定各种来源的流感病毒神经氨酸酶类型。

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