[紫外线照射灭活的甲型流感病毒的抗原特性]
[Antigenic properties of influenza virus type A inactivated by ultraviolet irradiation].
作者信息
Bokhnevich G M, Fridman E A, Peradze T V
出版信息
Vopr Virusol. 1978 Jan-Feb(1):74-9.
The effect of ultraviolet irradiation (UVI) on influenza A virus was studied. UVI of influenza A virus purified by adsorption chromatography on porous glass (PG) was found to result in irreversible inactivation of virus. The rate of inactivation directly depended upon the degree of virus purification. A reduction in the neuraminidase, hemagglutinating and antigenic activity of the virus occurred after a considerably longer UVI (over 7--10 min) than that required for inactivation of the purified virus (1--2 min) with the regimen of inactivation used. No changes in specificity of hemagglutinin and neuraminidase antigens after UVI were observed. The immunogenic activity of hemagglutinin and neuraminidase of the experimental lots of influenza vaccine purified on PG but inactivated by different means (UVI or formalin) was similar.
研究了紫外线照射（UVI）对甲型流感病毒的影响。发现通过多孔玻璃（PG）吸附色谱法纯化的甲型流感病毒经紫外线照射会导致病毒不可逆失活。失活速率直接取决于病毒的纯化程度。与使用的失活方案中纯化病毒失活所需的时间（1-2分钟）相比，在长得多的紫外线照射（超过7-10分钟）后，病毒的神经氨酸酶、血凝和抗原活性降低。紫外线照射后未观察到血凝素和神经氨酸酶抗原特异性的变化。在PG上纯化但通过不同方法（紫外线照射或福尔马林）灭活的实验批次流感疫苗的血凝素和神经氨酸酶的免疫原活性相似。
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