King D J, Hopkins S R
Avian Dis. 1983 Jan-Mar;27(1):100-12.
An infectious bronchitis virus (IBV) hemagglutination-inhibition (HI) test was used to assay serum-antibody titers after IBV vaccination of IBV-susceptible specific-pathogen-free broilers and commercial layers. Three-week-old broilers were vaccinated via eye-drop with IBV strains that represent the antigenic spectrum of commercial vaccines--Holland, Massachusetts 41 (41 Ms), Connecticut 46, Florida 18288, or JMK strain--and revaccinated 3 weeks later with either the same or a heterologous strain. Weekly serum samples were tested by IBV HI with homologous and heterologous antigens. Vaccinates, except for those vaccinated with the Holland strain, were HI-positive with homologous but not heterologous antigens by 1 to 2 weeks postvaccination. Sixteen-week-old IBV-vaccinated commercial layers were revaccinated with IBV Holland 52 (H 52) strain and subsequently infected with Arkansas 99 (Ark 99) and SE 17 strains. In contrast to the limited HI cross-reactivity of serum from IBV-vaccinated broilers, there were extensive cross-reactions in HI tests with 41 Ms, H 52, Ark 99, and SE 17 antigens of revaccinated layers. These results demonstrate that the IBV HI test is more strain-specific than previous reports indicate, especially when the test samples are from early postvaccination.
采用传染性支气管炎病毒（IBV）血凝抑制（HI）试验，对IBV易感的无特定病原体肉鸡和商品蛋鸡接种IBV疫苗后的血清抗体效价进行测定。对3周龄的肉鸡通过滴眼接种代表商业疫苗抗原谱的IBV毒株——荷兰株、马萨诸塞41株（41 Ms）、康涅狄格46株、佛罗里达18288株或JMK株，3周后用相同或异源毒株进行再次接种。每周采集血清样本，用同源和异源抗原进行IBV HI检测。除接种荷兰株的鸡外，接种疫苗的鸡在接种后1至2周时，同源抗原的HI检测呈阳性，而异源抗原的HI检测呈阴性。对16周龄接种IBV疫苗的商品蛋鸡用IBV荷兰52株（H 52）进行再次接种，随后用阿肯色99株（Ark 99）和SE 17株进行感染。与接种IBV疫苗的肉鸡血清有限的HI交叉反应不同，再次接种的蛋鸡血清在HI试验中与41 Ms、H 52、Ark 99和SE 17抗原存在广泛的交叉反应。这些结果表明，IBV HI试验比以前报道的更具毒株特异性，特别是当检测样本来自接种疫苗后的早期时。
