多聚腺苷酸形成受抑制后，从分离的肝细胞核中释放出的修饰信使核糖核酸
Modified messenger ribonucleic acid release from isolated hepatic nuclei after inhibition of polyadenylate formation.
作者信息
Schumm D E, Webb T E
出版信息
Biochem J. 1974 Apr;139(1):191-6. doi: 10.1042/bj1390191.
Abstract
Treatment of rats with Cordycepin (3'-deoxyadenosine), an inhibitor of nuclear poly(A) synthesis, selectively decreased the energy and cytosol-dependent release of a portion of the mRNA species from the isolated hepatic nuclei in a cell-free system by approximately 40%. An analysis of the differential binding of the transported mRNA containing poly(A) tracts to nitrocellulose filters, and to cellulose or poly(U)-Sepharose columns suggested that the poly(A) tracts in the mRNA transported from the isolated hepatic nuclei of Cordycepin-treated rats, are decreased in size. This size decrease was confirmed through an analysis of the average size of the poly(A) tracts, released from the messengers by ribonuclease activity, on sucrose density gradients.
摘要
用核多聚腺苷酸合成抑制剂虫草素（3'-脱氧腺苷）处理大鼠，在无细胞体系中，可使从分离的肝细胞核中释放的一部分mRNA种类的能量和胞质溶胶依赖性释放选择性降低约40%。对含有多聚腺苷酸尾的转运mRNA与硝酸纤维素滤膜、纤维素或聚尿苷酸-琼脂糖柱的差异结合分析表明，从经虫草素处理的大鼠分离肝细胞核转运的mRNA中的多聚腺苷酸尾大小减小。通过对核糖核酸酶活性从信使中释放的多聚腺苷酸尾在蔗糖密度梯度上的平均大小分析，证实了这种大小的减小。
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