克隆于pUB110的枯草芽孢杆菌α-淀粉酶基因启动子及氨基端信号肽区域的核苷酸序列。
Nucleotide sequence of the promoter and NH2-terminal signal peptide region of Bacillus subtilis alpha-amylase gene cloned in pUB110.
作者信息
Ohmura K, Yamazaki H, Takeichi Y, Nakayama A, Otozai K, Yamane K, Yamasaki M, Tamura G
出版信息
Biochem Biophys Res Commun. 1983 Apr 29;112(2):678-83. doi: 10.1016/0006-291x(83)91516-4.
The nucleotide sequence of the promotor and NH2-terminal signal peptide region of the alpha-amylase gene derived from the alpha-amylase hyperproducing strain B. subtilis NA64 was determined. DNA sequences of the NH2-terminal region of the mature alpha-amylase, 41 amino acid residues of the signal peptide, a Shine-Dalgarno sequence (AGGAG), a potential RNA polymerase recognition site (TTGAAA), and a potential Pribnow box (AAGTAA) were identified. The DNA sequence was quite different from that of the alpha-amylase gene of B. amyloliquefaciens.
测定了来自α-淀粉酶高产菌株枯草芽孢杆菌NA64的α-淀粉酶基因启动子和NH2-末端信号肽区域的核苷酸序列。鉴定出成熟α-淀粉酶NH2-末端区域的DNA序列、信号肽的41个氨基酸残基、一个Shine-Dalgarno序列（AGGAG）、一个潜在的RNA聚合酶识别位点（TTGAAA）和一个潜在的Pribnow框（AAGTAA）。该DNA序列与解淀粉芽孢杆菌的α-淀粉酶基因序列有很大不同。
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