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克隆于pUB110的枯草芽孢杆菌α-淀粉酶基因启动子及氨基端信号肽区域的核苷酸序列。

Nucleotide sequence of the promoter and NH2-terminal signal peptide region of Bacillus subtilis alpha-amylase gene cloned in pUB110.

作者信息

Ohmura K, Yamazaki H, Takeichi Y, Nakayama A, Otozai K, Yamane K, Yamasaki M, Tamura G

出版信息

Biochem Biophys Res Commun. 1983 Apr 29;112(2):678-83. doi: 10.1016/0006-291x(83)91516-4.

DOI:10.1016/0006-291x(83)91516-4
PMID:6189486
Abstract

The nucleotide sequence of the promotor and NH2-terminal signal peptide region of the alpha-amylase gene derived from the alpha-amylase hyperproducing strain B. subtilis NA64 was determined. DNA sequences of the NH2-terminal region of the mature alpha-amylase, 41 amino acid residues of the signal peptide, a Shine-Dalgarno sequence (AGGAG), a potential RNA polymerase recognition site (TTGAAA), and a potential Pribnow box (AAGTAA) were identified. The DNA sequence was quite different from that of the alpha-amylase gene of B. amyloliquefaciens.

摘要

测定了来自α-淀粉酶高产菌株枯草芽孢杆菌NA64的α-淀粉酶基因启动子和NH2-末端信号肽区域的核苷酸序列。鉴定出成熟α-淀粉酶NH2-末端区域的DNA序列、信号肽的41个氨基酸残基、一个Shine-Dalgarno序列（AGGAG）、一个潜在的RNA聚合酶识别位点（TTGAAA）和一个潜在的Pribnow框（AAGTAA）。该DNA序列与解淀粉芽孢杆菌的α-淀粉酶基因序列有很大不同。

相似文献

1
Nucleotide sequence of the promoter and NH2-terminal signal peptide region of Bacillus subtilis alpha-amylase gene cloned in pUB110.克隆于pUB110的枯草芽孢杆菌α-淀粉酶基因启动子及氨基端信号肽区域的核苷酸序列。
Biochem Biophys Res Commun. 1983 Apr 29;112(2):678-83. doi: 10.1016/0006-291x(83)91516-4.
2
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3
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引用本文的文献

1
Protein secretion in Bacillus species.芽孢杆菌属中的蛋白质分泌
Microbiol Rev. 1993 Mar;57(1):109-37. doi: 10.1128/mr.57.1.109-137.1993.
2
Alpha-amylase genes (amyR2 and amyE+) from an alpha-amylase-hyperproducing Bacillus subtilis strain: molecular cloning and nucleotide sequences.来自一株α-淀粉酶高产枯草芽孢杆菌菌株的α-淀粉酶基因（amyR2和amyE+）：分子克隆及核苷酸序列
J Bacteriol. 1983 Oct;156(1):327-37. doi: 10.1128/jb.156.1.327-337.1983.
3
Compilation of published signal sequences.已发表信号序列的汇编。
Nucleic Acids Res. 1984 Jul 11;12(13):5145-64. doi: 10.1093/nar/12.13.5145.
4
Length and structural effect of signal peptides derived from Bacillus subtilis alpha-amylase on secretion of Escherichia coli beta-lactamase in B. subtilis cells.源自枯草芽孢杆菌α-淀粉酶的信号肽的长度和结构对枯草芽孢杆菌细胞中大肠杆菌β-内酰胺酶分泌的影响
Nucleic Acids Res. 1984 Jul 11;12(13):5307-19. doi: 10.1093/nar/12.13.5307.
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Infect Immun. 1986 Apr;52(1):144-50. doi: 10.1128/iai.52.1.144-150.1986.
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Mol Gen Genet. 1989 Oct;219(1-2):129-36. doi: 10.1007/BF00261168.